CRISPR Screen Dataset
Giovannucci TA (2021) - 2-PMID34615851
Title: Inhibition of the ubiquitin-proteasome system by an NQO1-activatable compound.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : Mel JuSo [BTO:0001588, CELLOSAURUS:CVCL_1403]
- Phenotype : Response To Chemicals
- Condition : CBK77 (25 µM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 988 | Full Dataset Size: 19,364
- Experimental Setup : Drug Exposure | Duration: 5 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened the Brunello library for genes whose KO conferred resistance to CBK77
Score Distribution
< 0.99997
> 0.0000000000000417