CRISPR Screen Dataset
Wang X (2021) - 1-PMID34582788
Title: In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Breast Adenocarcinoma Cell Line [BTO:0001912]
- Cell Line : 4T1 [BTO:0004311, CELLOSAURUS:CVCL_0125]
- Phenotype : Cell Proliferation
- Library : MusCK | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 265 | Full Dataset Size: 4,721
- Experimental Setup : Timecourse | Duration: 10 Passages | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened a library of over 4,500 genes implicated in tumor initiation, progression, and immune modulation for essentiality in a breast cancer cell line
Score Distribution
< 1
> 0.00000000639