CRISPR Screen Dataset
Sheffer M (2021) - 3-PMID34253920
Title: Genome-scale screens identify factors regulating tumor cell responses to natural killer cells.
Screen Details:
- Screen Rationale : Cancer cell response to natural killer cells
- Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
- Cell Line : SW620 [BTO:0000675, CELLOSAURUS:CVCL_0547]
- Phenotype : Cell Proliferation
- Condition : NK (activated natural killer) cells
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,638 | Full Dataset Size: 20,114
- Experimental Setup : NK cell exposure | Duration: 72 Hours | MOI: = 0.4
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with p<= 0.05, enriched (or depleted) sgRNA >=2, and rank of enrichment or depletion <=2,000 are considered significantly enriched
- Genes with a significant positive MAGeCK score are enriched, suggesting KO of the hit gene confers resistance to NK cells, genes with a significant negative score are depleted, suggesting KO of the hit gene results in increased sensitivity to NK cells
- The authors used an E:T ratio selected to kill ~50% of the tumor cells for each cell line
Score Distribution
< 1
> 8.8e-17