CRISPR Screen Dataset
Ramaker RC (2021) - 15-PMID34049503
Title: Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Pancreatic Adenocarcinoma Cell Line [BTO:0001521]
- Cell Line : BxPC-3 [BTO:0001861, CELLOSAURUS:CVCL_0186]
- Phenotype : Response To Chemicals
- Condition : Irinotecan (250.0 nM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 179 | Full Dataset Size: 20,636
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased resistance to irinotecan in a BxPC-3 cell background
Score Distribution
< 2.422596993
> -7.581462461