CRISPR Screen Dataset

Ramaker RC (2021) - 14-PMID34049503

H. sapiens

Title: Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition.

Screen Details:

Screen Rationale : Increased drug resistance
Cell Type : Pancreatic Adenocarcinoma Cell Line [BTO:0001521]
Cell Line : BxPC-3 [BTO:0001861, CELLOSAURUS:CVCL_0186]
Phenotype : Response To Chemicals
Condition : Oxaliplatin (2.5 µM) [ChEBI]
Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 251 | Full Dataset Size: 20,647
Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Notes:

KO of the hit gene results in increased resistance to oxaliplatin in a BxPC-3 cell background

View More Screens from this Publication | Download Source Supplementary File

Score Distribution

< 2.078095923

> -3.695523945

CRISPR Screen Results (251 hits)

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