CRISPR Screen Dataset
Pusapati GV (2017) - 3-PMID29290584
Title: CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
Screen Details:
- Screen Rationale : Negative regulators of sonic hedgehog signalling pathway
- Cell Type : Embryonic Fibroblast Cell Line [BTO:0001958]
- Cell Line : NIH 3T3 [BTO:0000944, CELLOSAURUS:CVCL_0594]
- Phenotype : Regulation Of Signal Transduction Phenotype [CMPO:0000040]
- Condition : Sonic hedgehog protein (mouse) (25 nM) [Uniprot]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 2,383 | Full Dataset Size: 19,674
- Experimental Setup : Ligand Exposure | Duration: 24 Hours | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Screen for negative regulators of the Hedgehog signaling pathway
Score Distribution
< 1
> 0.000000239