CRISPR Screen Dataset
Gobbi G (2019) - 6-PMID31406246
Title: The Hippo pathway modulates resistance to BET proteins inhibitors in lung cancer cells.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Non-Small Cell Lung Adenocarcinoma Cell Line [BTO:0004078]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Cell Proliferation
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 1,275 | Full Dataset Size: 19,050
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Beta Score) < -0.5 AND Score.2 (FDR) < 0.05 OR Score.1 (Beta Score) > 0.5 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of genes with a negative score results in depletion, while a positive score indicated enrichment
Score Distribution
< 1.7621
> -3.1109