CRISPR Screen Dataset
Gobbi G (2019) - 1-PMID31406246
Title: The Hippo pathway modulates resistance to BET proteins inhibitors in lung cancer cells.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Non-Small Cell Lung Adenocarcinoma Cell Line [BTO:0004078]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Response To Chemicals
- Condition : JQ1 (1.0 µM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 147 | Full Dataset Size: 19,050
- Experimental Setup : Drug Exposure | Duration: 5 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Beta Score) > 0.5 AND Score.2 (FDR) < 0.05 OR Score.1 (Beta Score) < -0.5 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose loss conferred resistance (positive beta score) or sensitivity (negative beta score) to JQ1
Score Distribution
< 1.2492
> -1.2205