CRISPR Screen Dataset
Schleicher EM (2020) - 5-PMID33137164
Title: Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Response To Chemicals
- Condition : Berzosertib (VE822) (1.5 µM) [PubChem]
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : RSA | Number of Hits: 241 | Full Dataset Size: 18,885
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) < -2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Activation of the hit gene confers resistance to ATR inhibitor VE822
Score Distribution
< 1
> -6.2