CRISPR Screen Dataset
Schleicher EM (2020) - 4-PMID33137164
Title: Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : MCF-10A [BTO:0001939, CELLOSAURUS:CVCL_0598]
- Phenotype : Response To Chemicals
- Condition : AZD6738 (3.6 µM) [PubChem]
- Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : RSA | Number of Hits: 208 | Full Dataset Size: 18,885
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) < -2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Activation of the hit gene confers resistance to ATR inhibitor AZD6738
Score Distribution
< 1
> -4.722