CRISPR Screen Dataset
Morita K (2018) - 1-PMID30093494
Title: Genome-wide CRISPR screen identifies as a gene required for autophagosome formation.
Screen Details:
- Screen Rationale : Regulators of autophagy
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 21,686
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: ~ 0.6
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) <= 0.00032317 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in autophagy deficiencies.
Score Distribution
< 1
> 0.000000000000247