CRISPR Screen Dataset
Flynn RA (2021) - 14-PMID33743211
Title: Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : African Green Monkey Kidney Cell Line
- Cell Line : Vero-E6|VERO C1008 [BTO:0004755, COVOC:0020002, CELLOSAURUS:CVCL_0574]
- Phenotype : Response To Virus
- Condition : Virus: Influenza A virus (IAV: Orthomyxovirus A/WSN/1933)
- Library : CRISPRn Targeted Library (Flynn, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Delta Z scores | Number of Hits: 453 | Full Dataset Size: 890
- Experimental Setup : Virus Exposure | Duration: 7 Days | MOI: ~ 0.1
- Significance : Authors have indicated that results with Score.1 (Z-score) < 0.0 AND Score.2 (FDR) <= 0.001 OR Score.1 (Z-score) > 0.0 AND Score.2 (FDR) <= 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A custom mitochondrial mini-pool library was used that targeted 810 mitochondrial proteins to screen for the functional impact of factors that likely associate with SARS-CoV-2 RNA. The viral screen identified genes that confer resistance (pro-viral) or sensitization (anti-viral) when targeted by sgRNAs. These are indicated by a positive Z-score for enrichment (pro-viral genes knocked out) or depletion (anti-viral genes knocked out), respectively, with a conservative significance threshold of FDR =< 0.001.
Score Distribution
< 14.46554096
> -29.89879598