CRISPR Screen Dataset
Hisano Y (2019) - 1-PMID31147448
Title: Lysolipid receptor cross-talk regulates lymphatic endothelial junctions in lymph nodes.
Screen Details:
- Screen Rationale : Regulators of GPCR signaling
- Cell Type : Osteosarcoma Cell Line [BTO:0000407]
- Cell Line : U2OS [BTO:0001938, CELLOSAURUS:CVCL_0042]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : SAM v1 (Zeomycin) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 11 | Full Dataset Size: 23,728
- Experimental Setup : Timecourse | Duration: 2 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.9 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes encode proteins that enable S1PR1 coupling to Beta-arrestin
Score Distribution
< 1
> 0.0000303