CRISPR Screen Dataset
Koduri V (2021) - 1-PMID33547076
Title: Targeting oncoproteins with a positive selection assay for protein degraders.
Screen Details:
- Screen Rationale : Positive regulators of ASCL1
- Cell Type : T-lymphoblastic Leukemia Cell Line [BTO:0002504]
- Cell Line : Jurkat [BTO:0000661, CELLOSAURUS:CVCL_0065]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Targeted cancer-relevant druggable genes (CP1080, M-AB34) (Kaelin, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 7 | Full Dataset Size: 788
- Experimental Setup : Timecourse | Duration: 25 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.4 (q-Value) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- ASCL1-DCK* expression sensitized Jurkat cells to BVdU, cells were then infected with an sgRNA library and exposed to BVdU and screened for enriched sgRNAs, suggesting that the KO of the hit gene resulted in increased growth on BVdU and the gene normally results in increased ASCL1 levels
Score Distribution
< 7.173732884
> 0.429843499