CRISPR Screen Dataset
Balce DR (2021) - 1-PMID33372156
Title: UFMylation inhibits the proinflammatory capacity of interferon-γ-activated macrophages.
Screen Details:
- Screen Rationale : Negative regulators of the IFN pathway
- Cell Type : Microglial Cell Line [BTO:0003349]
- Cell Line : BV-2 [BTO:0003350, CELLOSAURUS:CVCL_0182]
- Phenotype : Protein/peptide Distribution [APO:0000209]
- Condition : Interferon gamma (2.0 units/mL)
- Library : Asagio (mouse) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 229 | Full Dataset Size: 17,448
- Experimental Setup : Cytokine exposure | Duration: 20 Hours
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 AND Score.2 (p-Value) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- authors screened for genes whose KO resulted in increased levels of iNOS (a classic marker for macrophage activation) suggesting the gene normally acts as a negative regulator of IFNgamma responses
Score Distribution
< 3.802125057
> -4.192252895