CRISPR Screen Dataset

Baggen J (2021) - 2-PMID33686287

H. sapiens

Title: Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2.

Screen Rationale : Increased resistance to virus
Cell Type : Hepatoma Cell Line [BTO:0000578]
Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
Phenotype : Response To Virus
Condition : Virus: HCoV-229E (Human coronavirus 229E) (0.5 MOI) [NCBI Taxid 11137]
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 7 | Full Dataset Size: 19,115
Experimental Setup : Virus Exposure | Duration: 18 Days | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

The high stringency screen involved infecting cells to identify pro-viral host genes that confer resistance under strong selection when knocked out. These are indicated by a positive score for enrichment. (Supplementary Table 5)

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