CRISPR Screen Dataset
Jia R (2019) - 1-PMID31692446
Title: Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3.
Screen Details:
- Screen Rationale : Regulators of autophagy
- Cell Type : Glioma Cell Line [BTO:0000711]
- Cell Line : H4 [BTO:0003704, CELLOSAURUS:CVCL_1239]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 917 | Full Dataset Size: 20,664
- Experimental Setup : Timecourse | Duration: 7 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used a reporter in which GFP accumulated when autophagy flux was disrupted and selected for cells with high GFP. These cells were examined for enriched sgRNAs indicating that KO of the hit gene resulted in decreased autophagy flux and that the gene normally participated in autophagy flux.
Score Distribution
< 1
> 0.000000000000253