CRISPR Screen Dataset
Cortez JT (2020) - 1-PMID32499641
Title: CRISPR screen in regulatory T cells reveals modulators of Foxp3.
Screen Details:
- Screen Rationale : Regulators of Foxp3 stability
- Cell Type : Regulatory T Cell [CL:0000815]
- Cell Line : primary Treg
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRn Nuclear Factor Targeted Library based on Brie sgRNAs | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 41 | Full Dataset Size: 493
- Experimental Setup : Timecourse | Duration: 8 Days
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.5 OR Score.4 (FDR) < 0.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened mouse primary Treg cells using a targeted library of ~490 nuclear factors based on guides from the Brie library. They sorted cells using a Foxp3 fluorescent reporter for cells with low Foxp3 expression.
- Genes whose KO resulted in enrichment in a low Foxp3 population (significant pos MAGECK score) suggest the gene normally functions as a positive regulator of Foxp3 levels
- Genes whose KO resulted in depletion in a low Foxp3 population (significant neg MAGECK score) suggest the gene normally functions as a negative regulator of Foxp3 levels
Score Distribution
< 1
> 0.000000137