CRISPR Screen Dataset
Kulsuptrakul J (2021) - 1-PMID33730579
Title: A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5.1 [BTO:0005463, CELLOSAURUS:CVCL_E049]
- Phenotype : Response To Virus
- Condition : Virus: Human hepatitis A virus HM175 (10.0 MOI) [TAXID:12098]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 206 | Full Dataset Size: 20,916
- Experimental Setup : Virus Exposure | Duration: 12 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased growth in the presence of Hepatitis A Vius HM175/18f
Score Distribution
< 1
> 9.97e-23