CRISPR Screen Dataset
Sharon DM (2020) - 1-PMID32699298
Title: A pooled genome-wide screening strategy to identify and rank influenza host restriction factors in cell-based vaccine production platforms.
Screen Details:
- Screen Rationale : Viral restriction factors
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293SF [CELLOSAURUS:CVCL_4V94]
- Phenotype : Response To Virus
- Condition : Virus: Influenza A (A/Puerto Rico/8/1934(H1N1) (0.1 MOI) [NCBI Taxid 211044]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 124 | Full Dataset Size: 19,107
- Experimental Setup : Virus Exposure | Duration: 40 Hours | MOI: = 2
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 AND Score.4 (Log2FC) > 0.4 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased presence of viral reporter in a FACs sorted population suggesting the gene normally functions as a viral restriction factor
Score Distribution
< 1
> 0.000000000000109