CRISPR Screen Dataset
Fomicheva M (2020) - 3-PMID33185187
Title: Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.
Screen Details:
- Screen Rationale : Positive regulators of density-dependent cell cycle arrest
- Cell Type : Breast Epithelium [BTO:0001428]
- Cell Line : EpH4 [CELLOSAURUS:CVCL_0073]
- Phenotype : Cell Cycle Progression [APO:0000253]
- Library : GECKO v2 (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 6 | Full Dataset Size: 22,619
- Experimental Setup : Timecourse | Duration: 42 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose KO resulted in proliferation despite high cell density, suggesting the gene normally contributes to density-dependent cell cycle arrest
Score Distribution
< 0.99999
> 0.0000000000033