CRISPR Screen Dataset
Burr ML (2019) - 2-PMID31564637
Title: An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer.
Screen Details:
- Screen Rationale : Negative regulators of MHC-I expression
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 99 | Full Dataset Size: 20,500
- Experimental Setup : Timecourse | Duration: 30 Days | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- The authors screened for genes whose loss induced MHC-I expression as determined by a reporter HLA-B antibody, suggesting the gene normally functions as a negative regulator of MHC-I expression
- The top 100 enriched sgRNA were selected
Score Distribution
< -2.488
> -9.204