CRISPR Screen Dataset
Lee J (2020) - 1-PMID33188726
Title: Genome-scale CRISPR screening identifies cell cycle and protein ubiquitination processes as druggable targets for erlotinib-resistant lung cancer.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : NCI-H820 [BTO:0006024, CELLOSAURUS:CVCL_1592]
- Phenotype : Response To Chemicals
- Condition : Erlotinib (1.0 µM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 81 | Full Dataset Size: 19,050
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors analyzed their screen data using MAGeCK and EdgeR and determined hits to be the genes that were significantly depleted in both analysis methods.
- Hit genes sensitized cells to erlotinib treatment
Score Distribution
< 4.3082
> -1.2516