CRISPR Screen Dataset
van de Weijer ML (2020) - 1-PMID32738194
Title: Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.
Screen Details:
- Screen Rationale : ERAD genes
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 16 | Full Dataset Size: 18,192
- Experimental Setup : Timecourse | Duration: 6 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) <= 2.05e-10 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes are those whose KO resulted in high levels of a GFP-tagged ERAD substrate reporter (CYP51A1TM), suggesting that the gene plays a role in proper ERAD function
Score Distribution
< 1
> 9.75e-16