CRISPR Screen Dataset
Clements KE (2020) - 1-PMID33257658
Title: Identification of regulators of poly-ADP-ribose polymerase inhibitor response through complementary CRISPR knockout and activation screens.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Response To Chemicals
- Condition : Olaparib (5 µM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 961 | Full Dataset Size: 19,113
- Experimental Setup : Drug Exposure | Duration: 4 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene causes decreased proliferation in the presence of Olaparib
Score Distribution
< 0.99997
> 0.000000462