CRISPR Screen Dataset

Li H (2021) - 1-PMID33110234

H. sapiens

Title: Genome-wide CRISPR screen identifies LGALS2 as an oxidative stress-responsive gene with an inhibitory function on colon tumor growth.

Screen Rationale : Increased/decreased resistance to oxidative stress
Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
Cell Line : HEK-293 [BTO:0000007, CELLOSAURUS:CVCL_0045]
Phenotype : Response To Chemicals
Condition : Hydrogen Peroxide (H2O2) (0.5 mM) [PubChem]
Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : CasTLE | Number of Hits: 973 | Full Dataset Size: 14,071
Experimental Setup : Drug Exposure | Duration: 6 Days | MOI: = 0.5
Significance : Authors have indicated that results with Score.3 (p-Value) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Genes with a negative CASTLE effect are depleted upon KO in response to oxidative stress. Genes with a positive CASTLE effect are enriched upon KO in response to oxidative stress.

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