CRISPR Screen Dataset
Thomsen EA (2020) - 1-PMID32585766
Title: Identification of BLNK and BTK as mediators of rituximab-induced programmed cell death by CRISPR screens in GCB-subtype diffuse large B-cell lymphoma.
Screen Details:
- Screen Rationale : Increased resistance to chimeric monoclonal antibody
- Cell Type : Diffuse Large B-cell Lymphoma Cell [BTO:0002521]
- Cell Line : OCI-Ly7 [BTO:0006414, CELLOSAURUS:CVCL_1881]
- Phenotype : Response To Chemicals
- Condition : Rituximab (10 µg/mL) []
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 44 | Full Dataset Size: 18,902
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes are genes whose loss confers increased resistance to a chimeric monoclonal antibody against CD20 (Rituximab) followed by short exposure to CDC (complement-dependent cytotoxicity) conditions in a diffuse large B-cell lymphoma cell line
Score Distribution
< 1
> 2.98e-20