CRISPR Screen Dataset
Zhang Y (2019) - 1-PMID31818950
Title: A -element within the locus mediates repression of expression via long-range chromatin interactions.
Screen Details:
- Screen Rationale : Regulators of p16/INK4A expression
- Cell Type : Lymphoma Or Leukaemia Cell Line [EFO:0002937]
- Cell Line : SEM [EFO:0002326, CELLOSAURUS:CVCL_0095]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRn Transcription Factor Targeted Library (Li, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 217 | Full Dataset Size: 1,739
- Experimental Setup : Timecourse | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- SEM cell line expressing a p16INK4A-P2A-mCherry reporter was used for screen
- Genes with a significant neg score (p<0.05) are positive regulators of p16/INK4A expression, genes with a significant positive score (p<0.05) are negative regulators of p16/INK4A expression
Score Distribution
< 1
> 0.0000000000043