CRISPR Screen Dataset
Fu X (2019) - 2-PMID31209294
Title: Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells.
Screen Details:
- Screen Rationale : Regulators of totipotency
- Cell Type : Embryonic Cell Line [EFO:0003040]
- Cell Line : Mouse Embryonic Stem Cell [EFO:0004038]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Dux gene induction
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 477 | Full Dataset Size: 20,646
- Experimental Setup : Other | Duration: 1 Days
- Significance : Authors have indicated that results with Score.2 (neg score p-value) < 0.01 OR Score.4 (pos score p-value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes whose KO results in a negative MAGeCK score associated with a significant p-value are depleted in the totipotent population, suggesting they are positive regulators of totipotency.
- Genes whose KO results in a positive MAGeCK score associated with a significant p-value are enriched in the totipotent population, suggesting they are negative regulators of totipotency.
Score Distribution
< 1
> 0.00000537