CRISPR Screen Dataset
Trinh MN (2020) - 1-PMID32690708
Title: Last step in the path of LDL cholesterol from lysosome to plasma membrane to ER is governed by phosphatidylserine.
Screen Details:
- Screen Rationale : LDL-derived cholesterol transport to the ER genes
- Cell Type : Immortal Cell Line [CLO:0000019]
- Cell Line : SV589j [CELLOSAURUS:CVCL_C8YJ]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 938 | Full Dataset Size: 19,112
- Experimental Setup : Timecourse | Duration: 16 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes prevents transport of LDL-derived cholesterol to the ER as measured by transcription of the LDLR protein by a fluorescent-tagged antibody
Score Distribution
< 1
> 0.00000000000000275