CRISPR Screen Dataset
Daniloski Z (2020) - 2-PMID33147445
Title: Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (0.3 MOI)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Robust-rank aggregation (RRA) | Number of Hits: 1,000 | Full Dataset Size: 19,049
- Experimental Setup : Virus Exposure | Duration: 6 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.1 (RRA rank) <= 1000.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The high viral dose screen involved infecting cells at a high MOI (0.3) to identify pro-viral host genes that confer resistance when knocked out. These are indicated by a positive score for enrichment. The authors identified ~1,000 genes with significant RRA enrichment (p < 0.05).
Score Distribution
< 19049
> 1