CRISPR Screen Dataset

Steinhart Z (2017) - 3-PMID27869803

H. sapiens

Title: Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.

Screen Rationale : Cell-essential genes
Cell Type : Pancreatic Adenocarcinoma Cell Line [BTO:0001521]
Cell Line : AsPC-1 [BTO:0001864, CELLOSAURUS:CVCL_0152]
Phenotype : Cell Proliferation
Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : BAGEL | Number of Hits: 936 | Full Dataset Size: 17,226
Experimental Setup : Timecourse | Duration: 20 Doublings | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 5.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Genes with a Bayes Factor (BF) above the threshold of 5.00 at an FDR of 5% (FDR < 0.05) were identified as fitness genes (hits) for this cell line in this CRISPR screen.

< 33.13

> -109.83