CRISPR Screen Dataset
Holmes DL (2020) - 1-PMID33122441
Title: A CRISPR-Cas9 screen identifies mitochondrial translation as an essential process in latent KSHV infection of human endothelial cells.
Screen Details:
- Screen Rationale : Decreased resistance to virus
- Cell Type : Microvascular Endothelial Cell Line [BTO:0003124]
- Cell Line : TIME [CELLOSAURUS:CVCL_0047]
- Phenotype : Response To Virus
- Condition : Virus: Human gammaherpesvirus 8 (Kaposi's sarcoma-associated herpesvirus KSHV) [NCBI Taxid 37296]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 146 | Full Dataset Size: 11,467
- Experimental Setup : Virus Exposure | Duration: 8 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in decreased growth in cells latently infected with KSHV
Score Distribution
< 0.99992
> 0.000045