CRISPR Screen Dataset
van den Boomen DJH (2020) - 1-PMID33144569
Title: A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.
Screen Details:
- Screen Rationale : Cellular cholesterol level maintenance genes
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 114 | Full Dataset Size: 20,564
- Experimental Setup : Timecourse | Duration: 18 Days
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- CRISPR knockout of these genes is predicted to induce spontaneous cholesterol depletion, SREBP2 activation and therefore HMGCS1-Clover (reporter) upregulation
Score Distribution
< 1
> 5.58e-34