CRISPR Screen Dataset
Ma H (2020) - 1-PMID33208938
Title: LDLRAD3 is a receptor for Venezuelan equine encephalitis virus.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Neuroblastoma Cell Line [BTO:0000932, EFO:0005214]
- Cell Line : Neuro-2a [BTO:0001976, CELLOSAURUS:CVCL_0470]
- Phenotype : Response To Virus
- Condition : Virus: Venezuelan equine encephalitis virus (VEEV) based chimera (SINV–VEEV–GFP) (1 MOI) [NCBI Taxid 11036]
- Library : GECKO v2 (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 56 | Full Dataset Size: 22,468
- Experimental Setup : Virus Exposure | Duration: 54 Hours | MOI: ~ 0.1
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes prevents infection by Venezuelan equine encephalitis virus as measured by expression of GFP after inoculation with a chimeric SINV-VEEV-GFP that encodes GFP
Score Distribution
< 1
> 0.000000080665