CRISPR Screen Dataset
Wei J (2020) - 12-PMID33147444
Title: Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : African Green Monkey Kidney Cell Line
- Cell Line : Vero-E6|VERO C1008 [BTO:0004755, COVOC:0020002, CELLOSAURUS:CVCL_0574]
- Phenotype : Response To Virus
- Condition : Virus: VSV-SARS-CoV-2-S (vesicular stomatitis virus expressing the SARS-CoV-2 spike) (0.1 MOI)
- Library : CRISPRn subpool library (CP1564) (Wilen, 2020) | Type: CRISPRn | Format: Pool | Enzyme: Cas9-v2 | Methodology: Knockout
- Analysis Method : Delta Z scores | Number of Hits: 225 | Full Dataset Size: 729
- Experimental Setup : Virus Exposure | Duration: 7 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.1 (Z-score) > 2.17 OR Score.1 (Z-score) < -2.17 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The viral screen identified genes that confer resistance (pro-viral) or sensitization (anti-viral) when targeted by sgRNAs, with a minimum FDR of 0.03 for non-targeting controls. These are indicated by a positive Z-score for enrichment (pro-viral genes knocked out) or depletion (anti-viral genes knocked out), respectively.
Score Distribution
< 14.37754888
> -15.41366482