CRISPR Screen Dataset
Steinhart Z (2017) - 1-PMID27869803
Title: Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Pancreatic Adenocarcinoma Cell Line [BTO:0001521]
- Cell Line : HPAF-II [BTO:0003266, CELLOSAURUS:CVCL_0313]
- Phenotype : Cell Proliferation
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 2,174 | Full Dataset Size: 17,226
- Experimental Setup : Timecourse | Duration: 20 Doublings | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 3.0 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- Genes with a Bayes Factor (BF) above the threshold of 3.00 at an FDR of 5% (FDR < 0.05) were identified as fitness genes (hits) for this cell line in this CRISPR screen.
Score Distribution
< 104.16
> -634.6