CRISPR Screen Dataset

Steinhart Z (2017) - 1-PMID27869803

H. sapiens

Title: Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.

Screen Rationale : Cell-essential genes (pancreatic adenocarcinoma)
Screen Type : Negative Selection | Format: Pool
Cell Line : HPAF-2 cell | Type: Pancreatic Adenocarcinoma Cell Line
Throughput : High Throughput
Enzyme : CAS9
Phenotype : Viability
Experimental Setup : Timecourse | Duration: 20 Doublings | MOI: = 0.3
Library : TKO (Toronto Knockout) v1 | Type: CRISPRn | Methodology: Knockout
Analysis Method : BAGEL | Number of Hits: 2,174 | Full Dataset Size: 17,226
Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 3 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Genes with a Bayes Factor (BF) above the threshold of 3.00 at an FDR of 5% (FDR < 0.05) were identified as fitness genes (hits) for this cell line in this CRISPR screen.

< 104.16

> -634.6