Screen Summary
Steinhart Z (2017) - 1-PMID27869803
Title: Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Screen Details:
- Screen Type : Negative Selection | Format: Pool
- Cell Line : HPAF-2 cell | Type: Pancreatic Adenocarcinoma Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Viability
- Experimental Setup : Timecourse | Duration: 20 Doublings | MOI: = 0.3
- Library : TKO (Toronto Knockout) v1 | Type: CRISPRn | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 2,174 | Full Dataset Size: 17,226
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a Bayes Factor (BF) above the threshold of 3.00 at an FDR of 5% (FDR < 0.05) were identified as fitness genes (hits) for this cell line in this CRISPR screen.
Score Distribution
< 104.16
> -634.6