CRISPR Screen Dataset

Sharon D (2019) - 1-PMID31666400

H. sapiens

Title: Inhibition of mitochondrial translation overcomes venetoclax resistance in AML through activation of the integrated stress response.

Screen Rationale : Increased/decreased drug resistance
Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
Cell Line : MOLM-13 [BTO:0004175, CELLOSAURUS:CVCL_2119]
Phenotype : Response To Chemicals
Condition : Venetoclax (400.0 nM) [ChEBI]
Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 6 | Full Dataset Size: 17,237
Experimental Setup : Drug Exposure | Duration: 8 Days
Significance : Authors have indicated that results with Score.2 (FDR) < 0.25 OR Score.4 (FDR) < 0.25 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

The authors developed a venetoclax resistance cell line (MOLM13-R1) and then transduced with TKOv1 to look for genes whose loss resulted in re-sensitization (genes with a negative score FDR<0.25) or increased resistance (genes with a positive score FDR<0.25)

< 1

> 0.0000000637