CRISPR Screen Dataset
Kim B (2019) - 2-PMID31038839
Title: Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate.
Screen Details:
- Screen Rationale : Microglia essential genes
- Cell Type : Microglial Cell Line [BTO:0003349]
- Cell Line : BV-2 [BTO:0003350, CELLOSAURUS:CVCL_0182]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 10 | Full Dataset Size: 19,672
- Experimental Setup : Timecourse | Duration: 9 Doublings
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.0005 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- CDr20- cell populations indicate KO of the gene results in loss of microglia as measured by the CDr20 fluorescent reporter
Score Distribution
< 0.99999
> 0.0000254