CRISPR Screen Dataset
Zhao Y (2020) - 2-PMID33189395
Title: Applying genome-wide CRISPR to identify known and novel genes and pathways that modulate formaldehyde toxicity.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Response To Chemicals
- Condition : Formaldehyde (150.0 µM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : EdgeR | Number of Hits: 971 | Full Dataset Size: 19,114
- Experimental Setup : Drug Exposure | Duration: 8 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (Log2) > 0.05 AND Score.2 (FDR) < 0.01 OR Score.1 (Log2) < -0.05 AND Score.2 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A CRISPR screen was carried out to identify candidate genes that increase sensitivity or resistance to formaldehyde when disrupted.
Score Distribution
< 3.26071173
> -7.7930377