CRISPR Screen Dataset
Chen X (2019) - 1-PMID31048321
Title: Targeting Mitochondrial Structure Sensitizes Acute Myeloid Leukemia to Venetoclax Treatment.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : MOLM-13 [BTO:0004175, CELLOSAURUS:CVCL_2119]
- Phenotype : Response To Chemicals
- Condition : Venetoclax (2.0 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Differential CRISPR score | Number of Hits: 680 | Full Dataset Size: 19,115
- Experimental Setup : Drug Exposure | Duration: 8 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) < -1.0 OR Score.1 (CRISPR Score (CS)) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose KO conferred resistance to venetoclax (positive CRISPR score) or sensitivity to venetoclax (negative CRISPR score)
Score Distribution
< 3.489426547
> -2.042944255