CRISPR Screen Dataset

Park JS (2019) - 1-PMID30590252

H. sapiens

Title: A FACS-Based Genome-wide CRISPR Screen Reveals a Requirement for COPI in Chlamydia trachomatis Invasion.

Screen Rationale : Genes required for C. trachomatis invasion
Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
Cell Line : HT-29 [BTO:0000182, CELLOSAURUS:CVCL_0320]
Phenotype : Response To Bacteria
Condition : Chlamydia trachomatis (50.0 MOI) [NCBI Taxid 813]
Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 18,647
Experimental Setup : Bacteria Exposure | Duration: 21 Hours
Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by 'Yes' in the HIT column of the table below.

The authors performed four replicates for genes whose loss prevented C. trachomatis invasion as measured by a fluorescent reporter. They report the replicate results separately, but considered hits to be those with a p-value<0.001 in at least two of the four replicates. Those hits have been designated for replicate 4 results.

< 0.99999

> 0.00000000000142