CRISPR Screen Dataset
Konermann S (2015) - 3-PMID25494202
Title: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Screen Details:
- Screen Rationale : Gene activation decreases fitness
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : A-375 [BTO:0002806, CELLOSAURUS:CVCL_0132]
- Phenotype : Cell Proliferation
- Library : SAM v1 (Zeomycin) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : RIGER | Number of Hits: 300 | Full Dataset Size: 23,430
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: < 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Score Distribution
< -1.449610591
> -2.992660039