CRISPR Screen Dataset
Lu A (2018) - 2-PMID30556811
Title: Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs.
Screen Details:
- Screen Rationale : Extracellular vesicle release effector genes
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Vesicle Distribution [APO:0000302]
- Library : CRISPR/Cas9-bEXOmiR (minus MMM sub-library) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 612 | Full Dataset Size: 18,508
- Experimental Setup : Timecourse | Duration: 48 Hours | MOI: < 1.0
- Significance : Authors have indicated that results with Score.2 (CasTLE Effect) <= -2.0 AND Score.4 (Log10 (p-value)) > 2.5 OR Score.2 (CasTLE Effect) >= 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a negative effect score show reduced extracellular bEXOmiR abundance upon deletion and normally act to acitvate EV production
- Genes with a positive effect score show increased extracellular bEXOmiR abundance upon deletion and normally act to suppress EV production
Score Distribution
< 63.5
> 0