CRISPR Screen Dataset
Schneider WM (2020) - 5-PMID33382968
Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5 [BTO:0004126, CELLOSAURUS:CVCL_7927]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2)|37ºC (0.1 MOI) [NCBI Taxid 2697049]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 147 | Full Dataset Size: 19,364
- Experimental Setup : Virus Exposure | Duration: 5 Days | MOI: = 0.21
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- KO of genes with a positive z-score show enriched sgRNA levels in the presence of SARS-CoV-2, suggesting loss of the gene are confers resistance against viral-induced cell death
- KO of genes with a negative z-score show depleted sgRNA levels in the presence of SARS-CoV-2, suggesting loss of the gene sensitizes cells to viral-induced cell death
Score Distribution
< 7.826371469
> -6.151932545