CRISPR Screen Dataset
Jacques S (2020) - 1-PMID32094149
Title: Imipridone Anticancer Compounds Ectopically Activate the ClpP Protease and Represent a New Scaffold for Antibiotic Development.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Pre-B Acute Lymphoblastic Leukemia Cell Line [BTO:0000603]
- Cell Line : NALM-6 [BTO:0003288, CELLOSAURUS:CVCL_0092]
- Phenotype : Response To Chemicals
- Condition : ONC201 (TIC10) (10.0 µM) [ChemSpider]
- Library : Extended Knockout Library (EKO) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RANKS | Number of Hits: 23 | Full Dataset Size: 19,008
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a positive RANKS score show increased resistance to ONC201 upon KO, genes with a negative RANKS score show increased sensitivity to ONC201 upon KO
Score Distribution
< 10.89
> -4.08