CRISPR Screen Dataset
Liu C (2019) - 1-PMID30862715
Title: Spindle Assembly Checkpoint Inhibition Can Resensitize p53-Null Stem Cells to Cancer Chemotherapy.
Screen Details:
- Screen Rationale : Genes whose KO re-sensitizes TP53 KO cells to cisplatin
- Cell Type : Embryonic Stem Cell Line [BTO:0001581]
- Cell Line : WA09 [BTO:0006062, CELLOSAURUS:CVCL_9773]
- Phenotype : Response To Chemicals
- Condition : Mutation: TP53 (ETG:7157) KO|Cisplatin (200 nM)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 137 | Full Dataset Size: 18,915
- Experimental Setup : Drug Exposure | Duration: 14 Doublings | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genetic interactions between TP53 and hit genes
- Genes whose KO re-sensitizes TP53 KO cells to cisplatin
Score Distribution
< 0.99982
> 0.0000268