CRISPR Screen Dataset
Hinze L (2019) - 1-PMID30991026
Title: Synthetic Lethality of Wnt Pathway Activation and Asparaginase in Drug-Resistant Acute Leukemias.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Lymphoma Or Leukaemia Cell Line [EFO:0002937]
- Cell Line : CCRF-CEM [BTO:0000736, CELLOSAURUS:CVCL_0207]
- Phenotype : Response To Chemicals
- Condition : Aspariginase (10.0 U/L)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 127 | Full Dataset Size: 21,699
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.0007 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased sensitivity to asparaginase
Score Distribution
< 1
> 0.0000000297