CRISPR Screen Dataset
Zimmermann M (2018) - 4-PMID29973717
Title: CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.
Screen Details:
- Screen Rationale : Genes whose KO restores resistance to PARP inhibitors in an RNASEH2 mutant
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Mutation: RNASEH2A (ETG0535) KO| Talazoparib (50 nM)
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 966 | Full Dataset Size: 17,237
- Experimental Setup : Drug Exposure | Duration: 18 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased proliferation in the presence of Talazoparib in an RNASEH2 mutant
Score Distribution
< 1
> 0.0000000000023