CRISPR Screen Dataset
Olivieri M (2020) - 12-PMID32649862
Title: A Genetic Map of the Response to DNA Damage in Human Cells.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Olaparib (5.0 µM) [PubChem]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 41 | Full Dataset Size: 17,277
- Experimental Setup : Drug Exposure | Duration: 18 Days | MOI: ~ 0.35
- Significance : Authors have indicated that results with Score.1 (Z-score) < -3.0 OR Score.1 (Z-score) > 6.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of genes with a negative Z-score confers sensitivity to drug KO of genes with a positive Z-score confers resistance to drug
Score Distribution
< 5.97
> -6.51